2005 Volume 53 Issue 1 Pages 95-96
Distribution of cystathionine γ-lyase activity was studied in fish. Liver homogenates were incubated with L homoserine at 25°C for 60 min, and 2-oxobutyrate formed by the enzymic reaction was determined and defined as the enzyme activity. Fish enzymes showed the maximum activities when the reactions were performed at pH 8.9 with Tris-HCl buffer. The highest enzyme activity was observed in smelt fish followed by that in yellowtail, and the lowest activity was found in carp. Addition of DL-propargylglycine or L-cysteine into the reaction mixtures inhibited 2-oxobutyrate productions, but the inhibition manners of cysteine were different among the examined species.
