EPIC-PCR for ribosomal protein gene intron: a case study in Thunnus tuna species

Abstract

Thirty-four exon-primed intron-crossing PCR (EPIC-PCR) primer pairs for amplifying ribosomal protein gene introns were tested using eight individuals from four Thunnus tuna species. Only two pairs failed to amplify fragments for all individuals. Whenever amplicons were observed, no apparent fragment length difference was observed between species. Possible allelic variation due to fragment length polymorphism was observed at 13 loci.