1965 Volume 14 Issue 12 Pages 651-657,712
In a previous paper it was shown with fluorescent antibody technic that nephrotoxin (anti-rat kidney immune rabbit sera), which is injected intravenously to a normal rat, lacalizes selectively in the glomerular basement membrane. In this report an experiment was designed to make nephrotoxin pass through glomerular basement membrane into tubular lumen to see if nephrotoxin reacts with tubular cytoplasm and its basement membrane. For this purpose nephrotoxin was injected to an aminonucleoside nephrotic rat and its kidney was subjected to the fluorescent staining with anti-rabbit-γ-globulin. Specific fluorescence were detected not only in glomerular basement membrane, but also in Bowman's urinary space and tubular lumen. But there were no specific fluorescence in tubular cytoplasm and its besement membrane. These results might give a clue to the problems concerning the common antigenicity between glomerular and tubular basement membrane, and other relating matters. In addition the distribution and intensity of rat-γ-globulin in gromeruli of aminonucleoside nephrosis were studied in this experiment to investigate whether autoimmune process concerns in the progress of this experimental nephrosis or not. The fluorescent staining with anti-rat-γ-globuin showed almost equal findings as a normal rat glomeruli and so-called "mesangial patterns" designated by Unanue & Dixon were not found in our experiment.
