1980 Volume 29 Issue 4 Pages 187-193
To investigate the applicability of the leukocyte adherence inhibition (LAI) test in patients with systemic lupus erythematosus (SLE), the tests were performed with peripheral blood leukocytes from 35 lupus patients by using antigens such as native DNA (n-DNA) and denatured DNA(d-DNA). An optimal antigen dose was 0.25mg/ml of DNA. This DNA concentration did not inhibit adherence of normal leukocytes. The cultured supernates from patients reactive to n-DNA inhibited the adherence of the leukocytes from a non-reactive patient. LAI indexes and positive rates of LAI test against n-DNA in SLE were significantly higher than normal individuals, but those against d-DNA were same as normal. LAI test results for n-DNA and d-DNA did not correlate. The LAI test did not distinguish between active and inactive lupus. Moreover, there was no significant correlation between LAI indexes and titers of anti-DNA antibody, serum complement levels or administered doses of steroid hormone. These results indicate that LAI tests against n-DNA may be useful in in vitro assay for cellmediated immunity in SLE.
