Abstract
We studied sterol biosynthesis and DNA synthesis in human peripheral mononuclear cell (PBMs), T cell enriched fraction, B cell enriched fraction and monocyte enriched fraction activated by various mitogens. l) The sterol biosynthesis was maximally accentuated at 36-48 hr from the start of culture in T cell mitogen activated PBMs, at 4 day in PWM activated PBMs and at 5 day in LPS activated PBMs, reapectively. And, the peak of sterol biosynthesis was earlier than that of DNA synthesis in all cases. 2) In the activation by T cell mitogens, sterol biosynthesis was accentuated in the T cell enriched and monocyte enriched fractions. And, in the former, DNA synthesis was accompanied and therefore, activated T cells were major factor of sterol biosynthesis. But, in the latter, DNA synthesis was not followed. So, it would be probable that sterol biosynthesis of monocytes is greater if they are activated by T cell mitogens. 3) In the activation by PWM and LPS, sterol biosynthesis in B cell enriched and monocyte enriched fractions was accentuated. And, in the former, DNA synthesis was followed. So, it would be that sterol biosynthesis of activated B cells is greater. And, in the monocyte enriched fraction, DNA synthesis was accepted. Therfore, it would be that in those fractions, activated B cells in place of monocytes are major factors of accentuation of sterol biosynthesis.
