1982 Volume 31 Issue 6 Pages 334-342
Methylcellulose culture of bone marrow cells from a child with multiple sulfatase dificiency (MSD) gave rise to colonies consisting of pure basphils. Distinction of the basophils from other cells was made by hitochemical and ultrastructual analysis. Linear relationship was seen between cultured marrow cells and basophil colonies. The peak velocity sedimentation rate of basophil colony forming cells was 5.6mm/hr and differed from other colony forming cells. These results showed that basophil colony forming cells in MSD appeared to be basophilic committed stem cells. Although the reason for basophil colony formation in MSD is unclear, myelogram and suspension culture study showed that this was not a reflection of increase in basophil precursors.
