Abstract
A relatively speedy purification of SRS-A was described. SRS-A was extracted using 80% ethanol. This was followed by isolation with column chromatographies using Amberlite XAD-7 and silica gel. Recovery of ^3H-leukotriene D_4 was 53.3%, and prostaglandin F_<2α>, E_2 and bradykinin were removed through these procedures. After this extraction and purification, SRS-A activity was measured by bioassay, using guinea pig ileum. Using this method, the inhibition induced by two β-stimulants, salbutamol and formoterol, on mite-allergen-induced SRS-A release from human lung tissue passively sensitized with allergic serum was compared. Both drugs inhibited the release of SRS-A in a dose-dependent manner. The concentrations of these drugs required for 50% inhibition were 1×10^<-12>M for formoterol and 3×10^<-9>M for salbutamol, respectively, indicating that formoterol was a more potent inhibitor than salbutamol for SRS-A release from the human lung.
