Evaluation of I nhibitory Synergism of Combination s of Monoterpeneson the G rowth of Cladosporium spp. and Aureobasidium spp.

Abstract

We observed inhibitory syn e rgism of the combinations of two kinds of monoterpenes containing ℓ- menthol on the growth of six strains of dematiaceous fungi ( Cladosporium spp. and Aureo basi- dium spp.) using direct contact experiment based on an agar medium dilution method.The mono- terpenes except ℓ- menthol are menthone, (R)-pulegone, linalool, (-)-carveol and citral. All of t he m are included in herb As for 0.02% (w/v) of ℓ- menthol concentration, the other mono- terpenes concentration was adjusted in 0.02-0.1% (v/v). For some strains, multiplier effects of antifungal activity to the fungi were observed in 0.05-0.1% (v/v) of (R)-pulegone, 0.1% (v/v) of linalool, 0.02% (v/v) of (-)-carveol, and 0.02% (v/v) of citral at 25° C. In other most combinations ,it was equal to the independent antifungal activity of ℓ-menthol, (-)- carveol or citral.