Abstract
A new species of the spider genus Anyphaena (Araneae: Anyphaenidae), Anyphaena yoshitakei sp. nov., is described on the basis of specimens obtained from the Ryukyu Islands, Japan. The male palpal structure of this species is similar to those of males of A. taiwanensis and A. pugil, but this species can be distinguished by the lack of palpal femur spines. The female can be easily distinguished from females of Asian congeneric species by the shape of the longitudinal middle furrow of the epigyne.