2013 Volume 18 Issue 4 Pages 205-209
Strain K-20T, a Gram-negative, nonmotile, nonspore-forming and strictly aerobic coccobacillus, which produces a pale pink pigment (R2A agar medium, 30℃, seven days) was isolated from a sample of biofilm obtained from a cooling tower in Tokyo, Japan. A phylogenetic analysis of the 16S rRNA partial gene sequences (1,439 bp) showed that the strain (accession number: AB297501) was related to Roseomonas frigidaquae CW67T and Roseomonas stagni HS-69T with 97.4% and 96.9% sequence similarity, respectively. Strain K-20T formed a distinct cluster with Roseomonas frigidaquae CW67T in the phylogenetic tree at a high bootstrap value (93%); however, distance was recognized between the strains. In addition, the DNA-DNA hybridization level between strain K-20T and Roseomonas frigidaquae JCM 15073T was 33%. The taxonomic data indicate that K-20T (=JCM 14634T =KCTC 32152T) should be classified in the genus Roseomonas as the type strain of a novel species, Roseomonas tokyonensis sp. nov.