Biocontrol Science
Online ISSN : 1884-0205
Print ISSN : 1342-4815
ISSN-L : 1342-4815
Note
Evaluation of a Rapid Oral Bacteria Quantification System Using Dielectrophoresis and the Impedance Measurement
KATSUHIKO HIROTASATORU INAGAKIRYO HAMADAKAZUYUKI ISHIHARAYOICHIRO MIYAKE
Author information
JOURNALS FREE ACCESS

2014 Volume 19 Issue 1 Pages 45-49

Details
Abstract

To clarify the availability of the dielectrophoretic impedance measurement (DEPIM) system as the evaluator for oral care, we evaluated the usefulness of DEPIM system by comparison with the standard plate counting (SPC) method. First, the relationship between the DEPIM results and bacterial concentration measured by SPC was clarified. Next, the measurement of the microorganism number in a mixed suspension was evaluated with DEPIM and SPC. The bacterial counts with DEPIM strongly correlated with those with SPC (r2=0.633-0.997) and this correlation was also shown in the measurement of a mixed bacterial suspension (ranging from 105 to 108 cfu/ml) of two bacterial species. Moreover, the experiments using dissociating enzymes to eliminate the influence of the size of the bacterial aggregates demonstrated that the microbial measurement results with DEPIM are unaffected by bacterial aggregates. This study demonstrated that bacterial counts with DEPIM strongly correlated with those with SPC and were unaffected by bacterial aggregates.

Information related to the author
© 2014 The Society for Antibacterial and Antifungal Agents, Japan
Previous article Next article
feedback
Top