Biocontrol Science
Online ISSN : 1884-0205
Print ISSN : 1342-4815
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The Development of a Novel, Validated, Rapid and Simple Method for the Detection of Sarcocystis fayeri in Horse Meat in the Sanitary Control Setting
MASATO FURUKAWAYASUTAKA MINEGISHISHINJI IZUMIYAMAKENJI YAGITAHIDETO MORITAKU UEMURAYOSHIKI ETOHERIKO MAEDAMARI SASAKIKAZUYA ICHINOSESEIYA HARADAYOICHI KAMATAMASAKI OTAGIRIYOSHIKO SUGITA-KONISHITAKAHIRO OHNISHI
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2016 Volume 21 Issue 2 Pages 131-134

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Abstract

 Sarcocystis fayeri (S. fayeri) is a newly identified causative agent of foodborne disease that is associated with the consumption of raw horse meat. The testing methods prescribed by the Ministry of Health, Labour and Welfare of Japan are time consuming and require the use of expensive equipment and a high level of technical expertise. Accordingly, these methods are not suitable for use in the routine sanitary control setting to prevent outbreaks of foodborne disease. In order to solve these problems, we have developed a new, rapid and simple testing method using LAMP, which takes only 1 hour to perform and which does not involve the use of any expensive equipment or expert techniques. For the validation of this method, an inter-laboratory study was performed among 5 institutes using 10 samples infected with various concentrations of S. fayeri. The results of the inter-laboratory study demonstrated that our LAMP method could detect S. fayeri at concentrations greater than 104 copies/g. Thus, this new method could be useful in screening for S. fayeri as a routine sanitary control procedure.

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© 2016 The Society for Antibacterial and Antifungal Agents, Japan
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