Biocontrol Science
Online ISSN : 1884-0205
Print ISSN : 1342-4815
ISSN-L : 1342-4815
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A New Protocol for the Detection of Sterigmatocystin-producing Aspergillus Section Versicolores Using a High Discrimination Polymerase
ATSUTAKA KUBOSAKINAOKI KOBAYASHIMAIKO WATANABETOMOYA YOSHINARIKOSUKE TAKATORIYUTAKA KIKUCHIYUKIKO HARA-KUDOJUN TERAJIMAYOSHIKO SUGITA-KONISHI
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2020 Volume 25 Issue 2 Pages 113-118

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Abstract

Aspergillus section Versicolores species, except Aspergillus sydowii, produce a carcinogenic mycotoxin sterigmatocystin (STC). Since these fungi are found in varied environmental milieu including indoor dust and food products, our aim was to develop a sensitive and convenient assay to detect STC producing fungal strains. We made use of a high discrimination DNA polymerase (HiDi DNA polymerase), for single nucleotide polymorphism (SNP)-based PCR amplification. Using specific primer pairs based on the SNPs between A. sydowii and other strains of Aspergillus section Versicolores, we succeeded in amplifying the genomic DNA all target strains except A. sydowii. These results confirm that the SNP-based PCR amplification technique, using a high discrimination DNA polymerase, was a reliable and robust screening method for target fungal strains.

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© 2020 The Society for Antibacterial and Antifungal Agents, Japan
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