Biocontrol Science
Online ISSN : 1884-0205
Print ISSN : 1342-4815
ISSN-L : 1342-4815
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Two Potential Probiotic Bacillus with Proteolytic Activity to Dietary Protein from Adult Feces
HAIQI WANGXICHUN ZHANGZHAOHUA CHENGENGXIN HAOGUILING LI
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2021 Volume 26 Issue 4 Pages 221-224

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Abstract

 Two Bacillus strains were screened and identified using 16S rRNA gene sequencing the phenotypic tests, and then characterized in vitro for the probiotic characteristics. They were able to tolerate pH 2.5 for 2.5 h, following 0.3% bile salts and 0.1% pancreatin treatment for 5 h. They exhibited good ability to attach to intestinal epithelial cells and were susceptible to most of the antibiotics and being killed by several. Further and more important, they showed good proteolytic activity to food protein as gelatin and milk, with even higher activity than the reference strain. Thus, these two Bacillus strains are considered as potential proteolytic probiotic strains to food proteins.

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© 2021 The Society for Antibacterial and Antifungal Agents, Japan
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