Abstract
Novel HNPP/Fast Red TR in situ hybridization method was used to study the response of river water microbial communities to aniline, as a model chemical pollutant, in two biodegradation test systems: a river die-away (TOC-HANDAI) test and acultivation (HANDAI) test. Pseudomonas rRNA group I and Acinetobacter were the most abundant groups during peak biodegradation activity in both test systems in spite of the differences in test conditions and initial community structures. Burkholderia-Pseudomonas rRNA III-authentic Alcaligenes group were significant only in the cultivation test under highly polluted conditions. Vibrio-Aeromonas and Flavobacterium-Cytophaga groups were adversely affected by aniline in both test systems.
