Metal-Mediated Control of the Activity of Bacillus subtilis Superoxide Dismutase

TAKASHI INAOKA; YOSHINOBU MATSUMURA; TETSUAKI TSUCHIDO

doi:10.4265/bio.4.55

Metal-Mediated Control of the Activity of Bacillus subtilis Superoxide Dismutase

TAKASHI INAOKA, YOSHINOBU MATSUMURA, TETSUAKI TSUCHIDO

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Keywords: Superoxide dismutase, Metal modulation, Post-translational regulation, Bacillus subtilis

JOURNAL FREE ACCESS

1999 Volume 4 Issue 1 Pages 55-58

DOI https://doi.org/10.4265/bio.4.55

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Published: March 20, 1999 Received: October 30, 1998 Available on J-STAGE: June 28, 2010 Accepted: November 26, 1998 Advance online publication: - Revised: -
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Date of correction: June 28, 2010 Reason for correction: - Correction: CITATION Details: Wrong : Beyer, W. F., Jr., and Fridovich, I.(1991) In vivo competition between iron and manganese for occupancy of the active site region of the manganesesuperoxide dismutase of Escherichia coll. J. Biol. Chem., 266, 303-308.
Carlioz, A., and Touati, D.(1988) Isolation of superoxide dismutase mutants in Escherichia coli: Is superoxide dismutase necessary for aerobic life? EMBO J., 5, 623-630.
Casillas-Martinez, L., and Setlow, P.(1997) Alkyl hydroperoxide reductase, catalase, MrgA, and superoxide dismutase are not involved in resistance of Bacillus subtilis spores to heat or oxidizing agents. J. Bacteriol. 179: 7420-7425.
Chen, L., Keramati, L., and Helmann, J, D.(1995) Coordinate regulation of Bacillus subtilis peroxide stress genes by hydrogen peroxide and metal ions. Proc. Natl. Acad. Sci. U. S. A., 92, 8190-8194.
Farr, S. B., and Kogoma, T.(1991) Oxidative stress responses in Escherichia coli and Salmonella typhimurium. Microbiol. Rev., 55, 561-585.
Hassan, H. M., and Schrum, L. M.(1994) Roles of manganese and iron in the regulation of the biosynthesis of manganese-superoxide dismutase in Escherichia coli. FEMS Microbiol. Rev. 14, 315-323.
Hassan, H. M., and Moody, C. S.(1987) Regulation of manganese-containing superoxide dismutase in Escherichia coli. J. Biol. Chem., 262, 17173-17177.
Henriques, A. O., Melsen, L. R., and Moran, C. P. Jr.(1998) Involvement of superoxide dismutase in spore coat assembly in Bacillus subtilis. J. Bacteriol., 180, 2285-2291.
Inaoka, T., Matsumura, Y., and Tsuchido, T.(1998) Molecular cloning and nucleotide sequence of the superoxide dismutase gene and characterization of its product from Bacillus subtilis. J. Bacteriol., 180, 3697-3703.
Inaoka, T, Matsumura, Y., and Tsuchido, T.(1999) SodA and manganese are essential for the resistance to oxidative stress in growing and sporulating cells of Bacillus subtilis. J. Bacteriol., 181.
McCord, J. M., and Fridovich, I.(1969) Superoxide dismutase: An enzymic function for erythrocuprein. J. Biol. Chem., 244, 6049-6055.
Privalle, C, T., and Fridovich, I.(1990) Anaerobic biosynthesis of the manganese-containing superoxide dismutase in Escherichia coll. J. Biol. Chem., 265, 21966-21970.
Privalle, C. T., and Fridovich, I.(1992) Transcriptional and maturational effects of manganese and iron on the biosyntheis of manganese-superoxide dismutase in Escherichia coll. J. Biol. Chem., 267, 9140-9145.

Right : Beyer, W. F., Jr., and Fridovich, I.(1991) In vivo competition between iron and manganese for occupancy of the active site region of the manganesesuperoxide dismutase of Escherichia coli. J. Biol. Chem., 266, 303-308.
Carlioz, A., and Touati, D.(1988) Isolation of superoxide dismutase mutants in Escherichia coli: Is superoxide dismutase necessary for aerobic life? EMBO J., 5, 623-630.
Casillas-Martinez, L., and Setlow, P.(1997) Alkyl hydroperoxide reductase, catalase, MrgA, and superoxide dismutase are not involved in resistance of Bacillus subtilis spores to heat or oxidizing agents. J. Bacteriol. 179: 7420-7425.
Chen, L., Keramati, L., and Helmann, J, D.(1995) Coordinate regulation of Bacillus subtilis peroxide stress genes by hydrogen peroxide and metal ions. Proc. Natl. Acad. Sci. U. S. A., 92, 8190-8194.
Farr, S. B., and Kogoma, T.(1991) Oxidative stress responses in Escherichia coli and Salmonella typhimurium. Microbiol. Rev., 55, 561-585.
Hassan, H. M., and Schrum, L. M.(1994) Roles of manganese and iron in the regulation of the biosynthesis of manganese-superoxide dismutase in Escherichia coli. FEMS Microbiol. Rev. 14, 315-323.
Hassan, H. M., and Moody, C. S.(1987) Regulation of manganese-containing superoxide dismutase in Escherichia coli. J. Biol. Chem., 262, 17173-17177.
Henriques, A. O., Melsen, L. R., and Moran, C. P. Jr.(1998) Involvement of superoxide dismutase in spore coat assembly in Bacillus subtilis. J. Bacteriol., 180, 2285-2291.
Inaoka, T., Matsumura, Y., and Tsuchido, T.(1998) Molecular cloning and nucleotide sequence of the superoxide dismutase gene and characterization of its product from Bacillus subtilis. J. Bacteriol., 180, 3697-3703.
Inaoka, T, Matsumura, Y., and Tsuchido, T.(1999) SodA and manganese are essential for the resistance to oxidative stress in growing and sporulating cells of Bacillus subtilis. J. Bacteriol., 181.
McCord, J. M., and Fridovich, I.(1969) Superoxide dismutase: An enzymic function for erythrocuprein. J. Biol. Chem., 244, 6049-6055.
Privalle, C, T., and Fridovich, I.(1990) Anaerobic biosynthesis of the manganese-containing superoxide dismutase in Escherichia coli. J. Biol. Chem., 265, 21966-21970.
Privalle, C. T., and Fridovich, I.(1992) Transcriptional and maturational effects of manganese and iron on the biosyntheis of manganese-superoxide dismutase in Escherichia coli. J. Biol. Chem., 267, 9140-9145.

Abstract

A Bacillus subtilis sodA recombinant plasmid, pMW-sod, was introduced into a Sod-deficient strain E. coli IM303 (sodA, sodB). The activity of superoxide dismutase in the crude extract of E. coli IM303 bearing pMW-sod was markedly increased by the addition of an inducer, isopropyl-β-D-thiogalactopyranoside, in Mn²⁺-supplemented medium; in contrast, it was kept at the basal level in Fe²⁺-supplemented medium. However, the specific activity of B. subtilis SodA purified from this strain was extremely low, compared with that from B. subtilis. An atomic absorption analysis revealed that a relatively high content of Fe²⁺ was bound to this purified B. subtilis SodA. We further demonstrated that this purified B. subtilis SodA was activated by incubation with manganese salt. These results suggest that B. subtilis SodA was posttranslationally regulated by the manganese in vivo and in vitro.

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