Bactericidal Activities of a Wet Disinfectant Mat in a Food Factory

SATORU TOYOSHIMA; YASUE TANIDA; KAZUHIKO YAMAMOTO; JUNNICHI YAMANAKA; TERUO KAMIKI; SADAO KOMEMUSHI

doi:10.4265/bio.7.101

SATORU TOYOSHIMA, YASUE TANIDA, KAZUHIKO YAMAMOTO, JUNNICHI YAMANAKA, TERUO KAMIKI, SADAO KOMEMUSHI

Author information

Keywords: Wetting disinfecting mat, Benzalkonium chloride, Sodium carbonate, Bactericidal activity, Morphological change

JOURNAL FREE ACCESS

2002 Volume 7 Issue 2 Pages 101-106

DOI https://doi.org/10.4265/bio.7.101

Details

Published: July 20, 2002 Received: March 29, 2001 Available on J-STAGE: June 28, 2010 Accepted: October 03, 2001 Advance online publication: - Revised: -
Correction information
Date of correction: June 28, 2010 Reason for correction: - Correction: CITATION Details: Wrong : Dulbecco, R., and Vogt, M.(1954) Plaque formation and isolation of pure lines with poliomyelitis viruses. J. Exp. Med., 99, 167-182.
Fukada, T., Shimizu, K., Kumada, T., Katahira, J., Kikuchi, K., Enari, T., and Ooki, Y.(1987) Antimicrobial adhesive mats and bacterial contamination (in Japanese). Jpn. J. Med. Instr., 57, 272-276.
Ishigaki, K., Kiyotani, M., and Ono, T.(1991) Measures against nosocomial infection in wards of internal medicine (in Japanese). Jpn. Soc. Environ. Infect., 6, 37-39.
Jono, K., and Higashide, E.(1986) Bacterial tolerance to benzalkonium chloride and chiorhexidine gluconate (in Japanese). J. Antibact. Antifung. Agents, 14, 541-550.
Jono, K., and Higashide, E.(1987) Bactericial activity of a new preparation of benzalkonium chloride mainly composed of dodecyldimethylbenzylammonium chloride (in Japanese). J. Antibact. Antifung. Agents, 15, 561-567.
Kourai, H.(1997) Mechanism of antibacterial action (in Japanese). In Kokin-no-Subete, pp.125-128, Sen-iPublishers, Osaka.
Komemushi, S., Fujita, T., Kamiki, T., and Yamagata, K.(1985) Microbial contamination of surfaces of new buildings and the efficacy of decontamination of these surfaces. J. Antibact. Antifung. Agents, 13, 151-160.
Miwatani, T.(1991) Management of drug resistant bacteria (in Japanese). In Handbook for Disinfectants, pp.61-67, Nissoken Publishers, Tokyo.
Nakahama, T., Kurokawa, K., and Fukushima, R.(1991) Measures against nosocomial infection with MRSA (in Japanese). Med. Drug J., 27, 2657-2662.
Nakasone, N., and Iwanaga, M.(1991) Purification and characterization of pili isolated from Vibrio parahaemolyticus Na 2. Infect. Immun., 59, 726-728.
Ogase, H.(1996) Reduction of air-borne MRSA and prevention of infection: Cleaning of environment in sickroom with air-circulation-type UV sterilizer.(in Japanese). Clean Technol., 3, 63-66.
Ogiri, M., Saitou, T., Arakawa, H., Shimizu, Y., Yoshida, A., Konno, S., Shikita K., and Takada, T.(1990) Contamination of the floor in a clean-hall-type surgery department (in Japanese). Jpn. Assoc. Ope. Med., 11, 74-77.
Sugano, Y., Sugawara, I., Iwabuchi, E., Fukuchi, M., ltou, Y., Nakano, S., Nakajima, T., Kohira, M., Masuda, Y., Saitou, J., Tanaka, A., Kamijyou, J., Hatakeyama, T., Adachi, K., and Inematsu, T.(1990) Examination of measures against nosocomial infection with MRSA: Preparation of bed environment (in Japanese) Jpn. Soc. Environ. Infect., 5, 89.
Takahashi, T., Matsumoto, F., Masuda, H., Takasaki, N., Tanifuji, M., Sakagami, Y., and Yokoyama, H.(1989) Bactericidal activities of disinfectants to methicillin resistant Staphylococcus aureus (M RSA) Jpn. Soc. Environ.Infect., 4, 25-31.
Watabe, S., lhara, I., and Okuda, K.(1994) Eliminating MRSA from the soles of nurses' shoes (in Japanese). Jpn. Soc. Environ. Infect., 9, 44-48.
Yamazaki, H., Sakagami, Y., Ochiai, C., Yokoyama, H., Masuda, H., Takahashi, T., Matsumoto, F., and Sakurai, I.(1990) Activities of antibiotics and disinfectants against Staphylococcus aureus isolated from hospital (in Japanese). J. Antibact. Antifung. Agents, 18, 263-272.

Right : Dulbecco, R., and Vogt, M.(1954) Plaque formation and isolation of pure lines with poliomyelitis viruses. J. Exp. Med., 99, 167-182.
Fukada, T., Shimizu, K., Kumada, T., Katahira, J., Kikuchi, K., Enari, T., and Ooki, Y.(1987) Antimicrobial adhesive mats and bacterial contamination (in Japanese). Jpn. J. Med. Instr., 57, 272-276.
Ishigaki, K., Kiyotani, M., and Ono, T.(1991) Measures against nosocomial infection in wards of internal medicine (in Japanese). Jpn. Soc. Environ. Infect., 6, 37-39.
Jono, K., and Higashide, E.(1986) Bacterial tolerance to benzalkonium chloride and chiorhexidine gluconate (in Japanese). J. Antibact. Antifung. Agents, 14, 541-550.
Jono, K., and Higashide, E.(1987) Bactericial activity of a new preparation of benzalkonium chloride mainly composed of dodecyldimethylbenzylammonium chloride (in Japanese). J. Antibact. Antifung. Agents, 15, 561-567.
Kourai, H.(1997) Mechanism of antibacterial action (in Japanese). In Kokin-no-Subete, pp.125-128, Sen-iPublishers, Osaka.
Komemushi, S., Fujita, T., Kamiki, T., and Yamagata, K.(1985) Microbial contamination of surfaces of new buildings and the efficacy of decontamination of these surfaces. J. Antibact. Antifung. Agents, 13, 151-160.
Miwatani, T.(1991) Management of drug resistant bacteria (in Japanese). In Handbook for Disinfectants, pp.61-67, Nissoken Publishers, Tokyo.
Nakahama, T., Kurokawa, K., and Fukushima, R.(1991) Measures against nosocomial infection with MRSA (in Japanese). Med. Drug J., 27, 2657-2662.
Nakasone, N., and Iwanaga, M.(1991) Purification and characterization of pili isolated from Vibrio parahaemolyticus Na 2. Infect. Immun., 59, 726-728.
Ogase, H.(1996) Reduction of air-borne MRSA and prevention of infection: Cleaning of environment in sickroom with air-circulation-type UV sterilizer.(in Japanese). Clean Technol., 3, 63-66.
Ogiri, M., Saitou, T., Arakawa, H., Shimizu, Y., Yoshida, A., Konno, S., Shikita K., and Takada, T.(1990) Contamination of the floor in a clean-hall-type surgery department (in Japanese). Jpn. Assoc. Ope. Med., 11, 74-77.
Sugano, Y., Sugawara, I., Iwabuchi, E., Fukuchi, M., ltou, Y., Nakano, S., Nakajima, T., Kohira, M., Masuda, Y., Saitou, J., Tanaka, A., Kamijyou, J., Hatakeyama, T., Adachi, K., and Inematsu, T.(1990) Examination of measures against nosocomial infection with MRSA: Preparation of bed environment (in Japanese) Jpn. Soc. Environ. Infect., 5, 89.
Takahashi, T., Matsumoto, F., Masuda, H., Takasaki, N., Tanifuji, M., Sakagami, Y., and Yokoyama, H.(1989) Bactericidal activities of disinfectants to methicillin resistant Staphylococcus aureus (MRSA) Jpn. Soc. Environ.Infect., 4, 25-31.
Watabe, S., lhara, I., and Okuda, K.(1994) Eliminating MRSA from the soles of nurses' shoes (in Japanese). Jpn. Soc. Environ. Infect., 9, 44-48.
Yamazaki, H., Sakagami, Y., Ochiai, C., Yokoyama, H., Masuda, H., Takahashi, T., Matsumoto, F., and Sakurai, I.(1990) Activities of antibiotics and disinfectants against Staphylococcus aureus isolated from hospital (in Japanese). J. Antibact. Antifung. Agents, 18, 263-272.

Abstract

The antibacterial activity of the agent of a wet disinfectant mat (WDM) utilizing benzalkonium chloride (BAC) and sodium carbonate was researched. The agent of the WDM showed a bactericidal activity in which there was a decrease of 10⁷cfu/ml or more in all the strains tested in an exposure of 30 s or more. On the WDM, Staphylococcus aureus showed blebs after 30 min, and remarkable destruction of bacteria body was also observed. After 2.5h on the WDM, the whole bacterial body of Salmonella enteritidis was covered with many irregular blebs. In the early stage at 5 min after pouring the bacterial suspension on the WDM, embedding of S.enteritidis in the drug layer was observed. These results suggested thatthe WDM would trap and kill microorganisms in food factories.

Corresponding author

Correction information

Register with J-STAGE for free!