Abstract
In poly (A)+ RNA extracted from a lactating goat mammary gland, mRNA of about
750 nucleotides was shown to encode pre α-lactalbumin by using in vitro translation and immunoprecipitation. From the total poly (A)+ RNA, the cDNA library was constructed using the Escherichia coli plasmid pUC18; it was screened with the oligodeoxyribonucleotide probe corresponding to the amino acid sequence of Trp60-Gln65 of goat α-lactalbumin. A plasmid containing almost full-length cDNA of goat pre α-lactalbumin, pGLA-1, was identified. The cDNA insert of pGLA-1 comprises 727 base pairs and contains the signal peptide and mature protein sequence.
