Purification and Characterization of Six Cytochromes P-450 from Hepatic Microsomes of Immature Female Rats

Abstract

Six rat hepatic cytochromes P-450, named P-450IF-1-6, were purified from hepatic microsomes of immature female rats by high-performance liquid chromatography with anion-exchange, cation-exchange, and hydroxylapatite columns. The purified forms, except for P-450IF-4, gave a single protein-staining band on SDS-polyacryl-amide gel electrophoresis, with a minimum molecular weight of 50, 000 for P-450IF-1, 49, 000 for P-450IF-2, 47, 000 for P-4501F-3, 53, 500 for P-4501F-5, and 54, 000 for P-450IF-6. The CO-reduced spectral maximum of these forms was at 450 nm for P-450IF-1, 448 nm for P-4501F-2, 451 nm for P-4501F-3, 449 nm for P-4501F-4, 449 nm for P-4501F-5, and 450 nm for P-450IF-6. All of these cytochromes had the low-spin state of heme in the oxidized form. P-450IF-4 had high metabolic activity for both benzphetamine and 7-ethoxycoumarin. P-4501F-5 had moderate activity toward 7-ethoxycoumarin. P-4501F-3 catalyzed the hydroxylation of testosterone at the 7a-position effectively, but the other forms did not hydroxylate testosterone. Analysis of the NH₂-terminal sequence showed that P-450IF-1, 2, 3, 5, and 6 differed structurally from each other. The sequences of P-450IF-1 and IF-2 were somewhat homologous, but the NH₂-terminal sequences of the other forms were all different. Based on these results, we concluded that P-450IF-1 corresponded to one of the phenobarbital-inducible forms in male rat liver. P-4501F-2 was a female-specific form and its concentration was low. P-4501F-5 and IF-6 were new forms not purified previously.