The Primary Structure of Chicken Muscle Acylphosphatase Isozyme Ch1

Abstract

The amino acid sequence of chicken muscle acylphosphatase isozyme Chl was determined. The protein consists of 102 amino acid residues, does not contain histidine, and the NH₂-terminus is acetylated: Ac-Ser-Ala-Leu-Thr-Lys-Ala-Ser-Gly-Se-Leu-Lys-Ser-Val-Asp-Tyr-Glu-Val-Phe-Gly-Arg-Val-GIn-Gly-Val-Cys-Phe-Arg-Met-Tyr-Thr-GIu-Glu-Glu-Ala-Arg-Lys-Leu-Gly-Val-Val-Gly-Trp-Val-Lys-Asn-Thr-Ser-GIn-Gly-Thr-Val-Thr-Gly-Gln-Val-Gln-Gly-Pro-Glu-Asp-Lys-Val-Asn-Ala-Met-Lys-Ser-Trp-Leu-Ser-Lys-Val-GIy-Ser-Pro-Ser-Ser-Arg-Ile-Asp-Arg-Thr-Lys-Phe-Ser-Asn-Glu-Lys-Glu-Ile-Ser-Lys-Leu-Asp-Phe-Ser-Gly-Phe-Ser-Thr-Arg-Tyr-OH. This sequence differs in 44% of the total positions from the other isozyme (Ch2) of chicken muscle acylphosphatase (Ohba _{et al}., the accompanying paper). The sequence of Chl has three substitutions from that of turkey muscle acylphospha-tase; these are Ser from Ala at position 9, Ser from Arg at 47, and Lys from Asn at 83. The sequence has about 80% homology with those of mammalian muscle acylphosphatases.