The Structure of the O-Polysaccharide from Pseudomonas aeruginosa IID 1009 (ATCC 27585)

Abstract

Structural studies were carried out on the O-polysaccharide fraction obtained by mild acid treatment of the lipopolysaccharide from Pseudomonas aeruginosa IID 1009 (ATCC 27585). TheO-polysaccharide was composed of L-rhamnose, N-acetyl-D-quinovosamine, and N-acetyl-L-galactosaminuronic acid in a molar ratio of 1 : 1 :1. The results from analysis of fragments obtained by hydrogen fluoride hydrolysis of O-polysaccharide, together with data on methylation analysis and nuclear magnetic resonance spectroscopic analysis, led to the most likely structure of the repeating units of the polymer chain →4)L-GalNAcA(αl →3)D-QuiNAc(α1 →3)L-Rha(α1 →, in which about 70% of the rhamnose residues were O- aeetylated at C-2. This structure coincides with that of the repeating unit of Lanyi 02 a, b polysaccharides.