Abstract
α2-Plasmin inhibitor (α2PI) was purified directly from human plasma by using a monoclonal antibody affinity column, which recognizes the reactive site of α2PI.α2PI was eluted from the column under mild conditions with 50% v/v ethyleneglycol containing 0.05% Tween 80 in phosphate-buffered saline, pH 7.4. The yield was around 50% and the specific activity of the purified protein was the same as that of the best product of preparation conventionally purified α2PI.
