1989 Volume 106 Issue 5 Pages 878-886
A novel sulfated glycosphingolipid that belongs to “globo-series” was isolated from human kidney. This lipid was purified from a pooled kidney preparation by chloroform-methanol extraction, mild alkaline treatment, DEAE-Sephadex and silicic acid column chromatographies, and preparative TLC. The structure and the properties were studied by IR spectroscopy, proton NMR spectroscopy, negative secondary ion-mass spectrometry, solvolysis, periodate oxidation, compositional and methylation analyses, monoclonal antibodies, and a sulfatide-binding protein. From the results of the above analyses, the structure of this glycolipid was proposed to be HSO3-3GalNAc β 1-3Gal α 1-4Gal β 1-4Glc β 1-1ceramide. This sulfated lipid reacted with a monoclonal anti-SSEA-3 (stage-specific embryonic antigen-3)(MC-631)(Kannagi, R., Cochran, N. A., Ishigami, F., Hakomori, S., Andrews, P. W., Knowles, B. B., & Solter, D.(1983) EMBO J. 2, 2355-2361), whose epitope is R-3GalNAc β 1-3Gal α 1-4Gal β 1-R', on TLC and solid-phase radioimmunoassay. This lipid also bound to the 125I-labeled sulfatide-binding protein, thrombospondin. The yield of this sulfated glycolipid was 34pmol/g of tissue, which was about 0.028, 0.16, and 18mol% of galactosyl- and lactosylceramide sulfates, and globopentaosylceramide sulfate (Nagai, K.-i., Roberts, D. D., Toida, T., Matsumoto, H., Kushi, Y., Handa, S., & Ishizuka, I.(1989) J. Biol. Chem. 264, in press), respectively, in human kidney.
