Identification and Distribution of Tropomyosin Isoforms in Chicken Digestive Canal

Abstract

Two-dimensional gel electrophoresis of eight digestive organ extracts from 60-day-old chicks was performed. Judging from the similarity of their protein maps, the organs were classified into the following four types: 1) esophagus type, 2) proventriculus type, 3) gizzard type, and 4) intestine type. In four representative organs of these types, the distribution of tropomyosin isoforms was examined, and four high- and five low-M_r-type isoforms in addition to α and β isoforms were detected in the embryonic organs. In the adult organs, however, there were three high- and four low-M_r-type isoforms, which were restricted to the mucous membrane, in addition to α and β isoforms. Immunoblot analysis indicated that the high- and low-M_r-type isoforms in the embryo corresponded with those in the adult mucous membrane, but differences in the number and amount of the isoforms were found between the embryo and the adult mucous membrane.