Augmentation of Prostaglandin E₂ Production by Mammalian Phospholipase A₂ Added Exogenously

Abstract

Rat group II phospholipase A₂ added exogenously to A23187-activated HL-60 granulocytes augmented their production of prostaglandin E₂. Human group II phospholipase A₂ and porcine group I phospholipase A₂ augmented the prostaglandin E2 production in a similar manner. No significant increase in prostaglandin E₂ production was observed when cells were treated with purified phospholipase A₂ in the absence of A23187. Extracellular phospholipase A₂ at inflamed sites may contribute to the generation of pro-inflammatory lipid mediators by hydrolyzing the cellular phospholipids of activated inflammatory cells.