The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Purification and Characterization of Genetically PolymorphicDeoxyribonuclease I from Human Kidney
Daita NadanoToshihiro YasudaKoichiro Kishi
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JOURNAL FREE ACCESS

1991 Volume 110 Issue 3 Pages 321-323

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Abstract
Deoxyribonuclease I (DNase I) was purified about 850, 000-fold from human kidney using a rabbit anti-human urine DNase I antibody and sensitive DNase I activity assay. On SDS-PAGE, the purified kidney DNase I gave a single major band, and its molecular mass was estimated to be 38, 000 Da. The activity of purified kidney DNase I was dependent on the presence of Mg2+ and Ca2+. G-Actin inhibited the activity, as did the anti-urine DNase I antibody. The properties of the kidney DNase I were the same as those of urine DNase I.
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© The Japanese Biochemical Society
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