Crystallization and Preliminary X-Ray Diffraction Study of Cytochrome c₅₅₂ from Hydrogenobacter thermophilus

Abstract

Cytochrome c₅₅₂ from a thermophilic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus, exhibits remarkable thermostability. The oxidized cytochrome c₅₅₂ has been crystallized in an ethanol/water mixture by means of the vapor diffusion method. The crystals belong to the orthorhombic system, space group P2₁2₁2, with unit cell dimensions of a=93.4 Å, b=52.9 Å, and c =32.4 Å. Most probably the asymmetric unit contains two molecules of cytochrome c₅₅₂. The crystals diffract X-rays to better than 2.5 Å resolution and are stable to X-ray irradiation.