Studies on the Uronic Acid-Containing Glycoproteins of Fusarium sp. M7-1: IV. Isolation and Identification of Four Novel Oligosaccharide Units Derived from the Acidic Polysaccharide Chain

Abstract

Four novel oligosaccharide units were isolated from the acetolysis products of the acidic polysaccharide chain derived from the glycoproteins of Fusarium sp. M7-1. Their chemical structures were resolved mainly by ¹H-NMR spectrometry in combination with methylation analysis and mass spectrometry. The results indicate that these oligosaccharide units originated from the side chains, GlcNAcα1→4GlcAα1→2(GlcNAcα1→4)GlcAα1→2Gal, GlcNAcα1→4GlcAα1→2 (GlcNAcα1→4) GlcAα1→2 (GlcNAcα1→4) GlcAα1→2Gal, ChN←P→ 6Manβ1→4GlcAα1→2Gal, and Manβ1→2 (ChN←P→6) Manβ1→4GlcAα1→2Gal linked together with the other units reported previously [Jikibara et al. (1992) J. Biochem. 111, 236-243] through β1→6galactofuranoside linkages in the acidic polysaccharide chain.