The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Major ATPases on Clofibrate-Induced Rat Liver Peroxisomes Are Not Associated with 70 kDa Peroxisomal Membrane Protein (PMP7O)
Sakae ShimizuTsuneo ImanakaTatsuya TakanoShoji Ohkuma
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1992 Volume 112 Issue 6 Pages 733-736


We previously reported that novel Mg2+-ATPases were induced in rat liver peroxisomes by clofibrate administration and that these activities consisted of at least two types of enzymes, N-ethylmaleimide (NEM)-sensitive and -resistant. Here we present evidence that neither of these major peroxisomal ATPases is associated with the 70-kDa peroxisomal membrane protein (PMP70), because: (i) proteinase K treatment of peroxisomes resulted in inactivation of only NEM-sensitive ATPase, whereas disappeared PMP70 completely; (ii) NEM-sensitive ATPase activity was barely immunoprecipitated with anti-PMP70 IgG; (iii) the solubilized ATPases behaved differently from PMP70 on native PAGE; and finally (iv), the major peroxisomal ATPases were separated from PMP70 on gel filtration chromatography.

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