Effects of Polyamines on a Continuous Cell-Free Protein Synthesis System of an Extreme Thermophile, Thermus thermophilus

Abstract

A continuous cell-free protein synthesis system of an extremely thermophilic eubacterium, Thermus thermophilus HB 27, was constructed. This system produced MS 2 phage RNA translation products at a rate of more than 5μg per hour per 1.9mg of ribosomes at 65°C and the production continued linearly for at least 340min. When no polyamine was added, the system did not produce the proteins. The highest activity was recorded when 0.1mM tetrakis (3-aminopropyl) ammonium and 1.0mM spermine were added simultaneously.