Abstract
To determine the distributions of two known dystrophin isoforms derived from the 3' part of the dystrophin gene and of the dystrophin-associated proteins [50 DAG (A 2) and 43 DAG (A 3 a)] by immunoblot analysis, we examined various monkey tissues [skeletal (quad-riceps), cardiac (left ventricle), and smooth (aorta and uterus) muscles, lung, liver, central nervous system (cerebrum, cerebellum, and spinal cord), and peripheral nerve (sciatic nerve)]. With the antibody against the C-terminal domain of dystrophin, we found three bands other than the 400-kDa band corresponding to dystrophin or utrophin. These are a 130-kDa band detected in central nervous system, a 110-kDa band in peripheral nerve and spinal cord, and a 70-kDa band in all the tissues examined except skeletal muscle and peripheral nerve. The 130-kDa band may be a novel protein not yet reported, while the last two bands may correspond to Dp 116 and Dp 71, respectively [Lederfein et al. (1992) Proc. Natl. Acad. Sci. USA 89, 5346-5350 and Byers et al. (1993) Nature Genet. 4, 77-81]. Dystro-phin-associated protein, 43 DAG, was detected in all the tissues examined, but 50 DAG was detected only in skeletal and cardiac muscles. Furthermore, a unique 70-kDa band reacting with anti-50 DAG antiserum was found in all the tissues other than skeletal muscle.
