1994 Volume 116 Issue 5 Pages 1123-1126
Bile acids extracted from the urine of a healthy volunteer who excreted 7β-hydroxylated bile acids were fractionated to nonamidated, glycine-conjugated, taurine-conjugated, and sulfated bile acid fractions. The chemical conjugation types of the 7β-hydroxylated bile acids were then determined by treatment with several enzymes and by capillary column gas chromatography. Large amounts of 3α, 7β, 12α-trihydroxycholanoic acid were present as nonamidated and nonconjugated bile acids, while 3β, 7β-dihydroxycholanoic acid formed nonamidated bile acid N-acetylglucosaminide. In addition, ursodeoxycholic acid formed both glycine-conjugated bile acid and glycine-conjugated bile acid N-acetylglucosaminide. Bile acid N-acetylglucosaminides were hydrolyzed by solvolysis.