Crystallization and Preliminary X-Ray Analysis of Cytochrome c-552 from Nitrosomonas europaea

Chikahiro Nagata; Hideaki Moriyama; Taketomo Fujiwara; Yoshihiro Fukumori; Nobuo Tanaka

Crystallization and Preliminary X-Ray Analysis of Cytochrome c-552 from Nitrosomonas europaea

Chikahiro Nagata, Hideaki Moriyama, Taketomo Fujiwara, Yoshihiro Fukumori, Nobuo Tanaka

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Keywords: crystallization, cytochrome c, electron transfer, chemoautotoroph, X-ray diffraction

JOURNAL FREE ACCESS

1994 Volume 116 Issue 5 Pages 946-947

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Abstract

Cytochrome c-552 from Nitrosomonas europaea was crystallized by the sandwiched drop, vapor diffusion method, with anmmonium sulfate as the precipitant. The crystals were found to belong to the space group P2₁, 2₁, 2₁, having unit cell dimensions of a=106.1 Å, b=126.1 Å, and c=57.7 Å. The crystals diffracted X-rays at greater than 3.0 Å resolution.

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