1996 Volume 119 Issue 2 Pages 246-251
The metabolism of diacetyl (2, 3-butanedione), acetoin (3-hydroxy-2-butanone), and 2, 3-butanediol, which are metabolites of acetaldehyde was quantitatively investigated using rat liver homogenate, liver perfusion, and in vivo experiments. Diacetyl and acetoin were reduced to 2, 3-butanediol in these experiments, but acetoin and 2, 3-butanediol were scarcely oxidized to diacetyl, indicating that the reduction reaction to 2, 3-butanediol from diacetyl occurs actively in rat liver. The formation of acetoin from diacetyl required either NADH or NADPH as a reductant, while the reduction of acetoin to 2, 3-butanediol required NADH. Acetoin and 2, 3-butanediol were more readily accumulated than diacetyl in brain tissue.