Crystallization and Preliminary X-Ray Diffraction Studies of a Rice Cysteine Proteinase Inhibitor, Oryzacystatin-I

Abstract

Oryzacystatin-I from rice seeds was overexpressed in Escherichia coli, purified, and crystallized by the sitting-drop vapor diffusion method. Crystals obtained with 2-methyl-2, 4-pentanediol as a precipitant exhibited space group I 4₁22, with unit cell parameters of a=b=100.0 Å, c=54.2 Å, and diffracted up to 2.8 Å resolution at 100 K. The crystals have one molecule per asymmetric unit.