Crystallization and Preliminary X-Ray Diffraction Studies of a Replication Initiator Protein (RepE54) of the Mini-F Plasmid Complexed with Iteron DNA

Abstract

A replication initiator protein (RepE54) complexed with iteron DNA at its binding site was crystallized by the hanging drop vapor diffusion method. The crystals belong to monoclinic space group C2 with unit cell dimensions of a=108.4 Å, b=81.9 Å, c=73.9 Å, and β=121.5°, where one molecule of the protein-DNA complex exists per asymmetric unit. They diffract X-rays up to 2.6 Å resolution with synchrotron radiation.