Effects of Missense Mutations PhellOIle and Glu244Asp in Human Cardiac Troponin T on Force Generation in Skinned Cardiac Muscle Fibers

Abstract

The functional effects of two missense mutations in human cardiac troponin T, PhellOlle and Glu244Asp, associated with familial hypertrophic cardiomyopathy were examined by exchanging the bacterially expressed and purified mutant troponin T into rabbit cardiac skinned muscle fibers. Both mutations significantly increased the maximum force without affecting the cooperativity. The Glu244Asp mutation also increased the Call sensitivity of the force generation, as in the case of other mutations associated with a poor prognosis. On the other hand, the PhellOlle mutation, associated with a favorable prognosis, had no effect on the Ca²⁺ sensitivity. The results strongly support the hypothesis that increased Call sensitivity is responsible for the pathogenesis of hypertrophic cardiomyopathy with a poor prognosis caused by mutations in troponin T.