Abstract
1. The enzyme which is responsible for the production of methylxlyoxal from hexosephosphate can be easily separated from the obstinately accompanying glyoxalase by dialysing the animal tissues or treating them with alcohol. These methods are preferable to the familiar autolysis-method in that they save much time. Particularly the dialysis-method has proved to be the most desirable one, as it destroys glyoxalase activity completely within several hours.
2. The optimum conditions for the production of methylglyoxal from hexosephosphate are studied. The use of the smallest possible quantity of enzyme and substrate is one of the essential factors.
3. When the autolysed or dialysed animal tissues are incubated with hexose-di-phosphate under the optimum conditions, almost 100% conversion of hexosephosphate to methyhlyoxal is achieved.
