A New Approach for Rapidly Reshaping Single-Chain Antibody In Vitro by Combining DNA Shuffling with Ribosome Display

Abstract

Antibody reshaping is an effective way to reduce the immunogenicity while maintaining or improving the affinity of murine antibodies. This paper describe a new in vitro approach for rapidly reshaping murine antibodies by combining DNA shuffling with ribosome display. With the new method, a reshaping anti-4-1 BB single-chain antibody (scFv), Re-4B4-1 scFv, which bound to its antigen (4-1 BB) specifically and strongly, was selected from a reshaping library. These results proved definitely the feasibility of the new designed approach for antibody reshaping.