Establishment of Stable hFis 1 Knockdown Cells with an siRNA Expression Vector

Ryoichi Arai; Kaori Ito; Motoaki Wakiyama; Eiko Matsumoto; Ayako Sakamoto; Yuuka Etou; Makiko Otsuki; Mio Inoue; Yoshikide Hayashizaki; Makoto Miyagishi; Kazunari Taira; Mikako Shirouzu; Shigeyuki Yokoyama

doi:10.1093/jb/mvh139

Ryoichi Arai, Kaori Ito, Motoaki Wakiyama, Eiko Matsumoto, Ayako Sakamoto, Yuuka Etou, Makiko Otsuki, Mio Inoue, Yoshikide Hayashizaki, Makoto Miyagishi, Kazunari Taira, Mikako Shirouzu, Shigeyuki Yokoyama

Author information

Ryoichi Arai
Protein Research Group RIKEN Genomic Sciences Center
Structurome Research Group RIKEN Harima Institute at SPring-8
Kaori Ito
Protein Research Group RIKEN Genomic Sciences Center
Motoaki Wakiyama
Protein Research Group RIKEN Genomic Sciences Center
Eiko Matsumoto
Protein Research Group RIKEN Genomic Sciences Center
Ayako Sakamoto
Protein Research Group RIKEN Genomic Sciences Center
Yuuka Etou
Protein Research Group RIKEN Genomic Sciences Center
Makiko Otsuki
Protein Research Group RIKEN Genomic Sciences Center
Mio Inoue
Protein Research Group RIKEN Genomic Sciences Center
Yoshikide Hayashizaki
Genome Exploration Research Group, RIKEN Genomic Sciences Center
Makoto Miyagishi
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
Gene Function Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Central 4
Kazunari Taira
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
Gene Function Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Central 4
Mikako Shirouzu
Protein Research Group RIKEN Genomic Sciences Center
Structurome Research Group RIKEN Harima Institute at SPring-8
Shigeyuki Yokoyama
Protein Research Group RIKEN Genomic Sciences Center
Structurome Research Group RIKEN Harima Institute at SPring-8
Structural and Molecular Biology Laboratory, RIKEN Harima Institute at SPring-8
Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo

Keywords: Fis 1, knockdown, mitochondrial fission, RNAi, siRNA

JOURNAL FREE ACCESS

2004 Volume 136 Issue 4 Pages 421-425

DOI https://doi.org/10.1093/jb/mvh139

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Abstract

Yeast Fis1p participates in mitochondrial fission, together with Dnm1p and Mdv1p. Recently, human Fis 1 (hFis 1) was reported to be involved in mitochondrial fission, together with Drp 1. We established stable transformants with an hFis 1 siRNA expression vector. In the stable hFis 1 knockdown cells, hFis 1 expression was suppressed to approximately 10%, and mitochondrial fission, induced by cisplatin treatment, was delayed. In addition, mouse Fis 1 (mFis 1) expression promoted mitochondrial fission and cell death in the hFis 1 knockdown cells, suggesting that mFis 1 complements the function of hFis 1. These hFis 1 siRNA expression vectors may be useful for studying the molecular function of mammalian Fis 1.

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