Abstract
Each phase of the reactions involved in the Embden-Meyerhof theory on the production of lactic acid from hexosedi-phosphoric acid by muscle tissue, was investigated with the employ-ment of Kato spindle cell sarcoma of rabbit instead of muscle. The results obtained are as follows.
1. Triosephosphoric acid was produced from hexosediphos-phoric acid by tumour tissue. The velocity of the reaction was. however, slower than that of muscle tissue.
2. Tumour tissue was unable to dismutate two molecules of triosephosphoric acid into phosphoglyceric and glyceroiphosphorie acids.
3. The decomposition of phosphoglyceric acid into pyruvic acid was not carried out by tumour tissue, the reaction being stopped at the stage of phosphopyruvic acid. However, pyruvic acid was easily produced from phosphopyruvic acid in the presence of adenosinetriphosphoric acid and adenylicc acid, or phosphatase.
4. The dismutation of pyruvic acid into lactic acid in the presence of α-glycerolphosphoric acid was not observable in tumour tissue.
5. In spite of the facts mentioned above that the process of the glycolysis of the Embden-Meyerhof hypothesis was stopped at several phases, a small amount of lactic acid was always produced from hexosediphosphoric acid by the tumour slices. The mechanism of this reaction is quite obscure at present. This reaction was inhibited only to a relatively slight degree by the quantities of fluoride or monoiodoacetic acid which completely stopped the production of lactic acid from glucose.
