A METHOD FOR DETERMINING SUGAR AND GLYCOGEN IN THE TISSUES BY ORCINOLSULPHURIC ACID METHOD

Abstract

1. A method is described for the determination of various sugars (glycogen, free true sugar and hydrolysable sugar) in the tissues at the same time.
2. The method described here involves the principles of the Pflüger procedure for the isolation of glycogen and the determination of sugars (true sugar, hydrolytic sugar and glycogen), using the orcinol sulphuric acid method.
3. By the previously published methods it was possible to estimate the amount of free sugar and glycogen, but it was impossible to estimate the amount of hydrolysable sugar.
4. Compared with the other methods, this method requires a shorter time for the determination of biological materials, and this procedure is more convenient and simpler to use than the breast method, requiring only a small amount of material.
5. The results obtained by this method show that the glycogen content of rabbits differ greatly with each individual rabbit; but the amount of free sugar and hydrolysable sugar in the tissue does not differ greatly, In the case of rabbits in fasting, their liver tissues showed the greatest decrease in glycogen content, accompanied by a marked decrease in free sugar and a slight decrease in hydrolysable sugar. There was no change in the amount of the hydrolysable sugar in the rabbit muscles.