A METHOD OF DETERMINING SUCROSE CONTENT IN THE BLOOD
1941 Volume 34 Issue 3 Pages 371-384
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1941 Volume 34 Issue 3 Pages 371-384
1. A method has been described for the determination of the sucrose content in the blood. It requires only 1 cc. blood.
2. This method may be used either for a study of sucrose metabolism, or for a sucrose tolerance test for clinical purpose. By this method the sucrose content in the blood can be quickly, easily and accurately estimated.
3. A certain factor influencing the conversion of sucrose has been studied, with the results that a complete conversion without destroying sugar may be carried out, when 0.5 cc. of 44g/dl H2SO4, with heating time of 5-10 minutes in temperature 100°C, is employed.
4. The author has ascertained this method to be not only quicker and simpler method but also equally accurate as the fermentation method, by estimating the fermentable sugar values before and after hydrolysis, and by the additional tests in which known amounts of sucrose have been added.
5. An intravenous injection of 1.5gm. sucrose solution per Kg. into rabbits immediately produces a sharp rise of blood-sucrose, which is followed by a fall 2 hours after the injection.
The subcutaneous injection of 2gm. sucrose solution per Kg. tends to increase the blood-sucrose content 15 minutes after the injection, and 1/2-1 hour after the injection it reaches to a maximum which lasts for almost 2 hours, followed by a fall to half the maximum value.
The intravenous injection of sucrose solution into rabbits produces no effect on the blood glucose figure. The effect on the blood-sucrose figure by the oral administration of 2.5gm. sucrose solution per Kg. to rabbits is also quite similar to that obtained by the subcutaneous administration, but the rise is less and a considerable increase is observed an hour after the administration.
The rapid and sharp rise of the blood-glucose value becomes apparent after 30 minutes and is maintained for 3 hours after the oral administration.
In concluding this report, I desire to express my deepest gratitude to Director M. Takeda for the kindness and constant encouragement he has manifested in directing this study, and to Prof. T. Nagayama (Prof. of Tokyo Jikei-Kwai Medical College) for the courtesy he has shown in persuing this paper.
