PROTEIN SYNTHESIS IN EXCISED SILKGLAND OF SILKWORM. I
1955 Volume 42 Issue 3 Pages 285-294
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1955 Volume 42 Issue 3 Pages 285-294
1. A method was described for measuring the formation of protein by silkgland of silkworm in vitro.
2. The optimum pH for the reaction of protein formation by silkgland was found to be between 7.8 and 8.5, with a peak at pH 8.0.
3. The reaction was stimulated by the addition of inorganic phosphate.
4. The effect of various substances were studied. A marked stimu-lation was observed with a-keto acids (such as pyruvate, oxalacetate, a-ketoglutarate), lactate, pantothenic acid and ascorbic acid.
5. NaF and 2, 4-dinitrophenol were found to inhibit the reaction.
6. The protein formed gave a purple biuret reaction and was precipitated by mercuric chloride, trichloracetic acid, picric acid, and alcohol.
7. From the above observations it is suggested that silkgland tissues synthesize protein in vitro under suitable conditions.
