Abstract
1. In extending our previous studies a number of hydroxy compounds were tested for their acceptor function in the enzymatic transglycosylation using β-glucosidase and β-galactosidase preparations from elder (Sambucus Sieboldiana) leaves and apricot emulsin.
2. It was found that most of aliphatic alcohols were able to act as the acceptor of β-glucosyl and β-galactosyl groups, while lactate and malate were inactive in this reaction.
3. In respect of either β-glucosidase or β-galactosidase the efficiency of various alcohols as acceptor in the transglycosylation differred between the enzyme of elder leaves and that of apricot emulsin, indicating that the acceptor specificity of the enzyme catalyzing transglycosylation may differ with the source of the enzyme.
The author wishes to express his sincere thanks to Professor T. Miwa for constant encouragement and guidance while this work was being pursued.
The present work was supported in part by a grant in Aid for Fundamental Scientific Research given to T. Miwa from the Ministry of Education.
