The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
STABILITY OF NUCLEIC ACID SYNTHESIZED IN THE PRESENCE OF CHLORAMPHENICOL IN E. COLI B UNDER GROWING AND RESTING CONDITIONS
TADAO HORIUCHISUMIKO SUNAKAWADEN-ICHI MIZUNO
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1958 Volume 45 Issue 11 Pages 875-883

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Abstract
1. The stability of the RNA and DNA synthesized in the presence of CM were examined using E. coli B and its auxotrophic mutants : tryptophan-requiring (A-5) and uracil or cytosine-requiring (F-2). It has been shown that these RNA and DNA were conserved under growing condition in the casamino acids medium.
2. This RNA was degraded by 50 per cent or more under resting condition where tryptophan and uracil were limiting factors using A-5 and F-2, respectively, but the conservation of this DNA was observed under the same condition.
3. This RNA was stabilized by the exposure to a growing stage during the lag time of bacterial growth, and after that this RNA showed no degradation even in case the limiting factor was removed.
4. The stability of the RNA between the growing and the resting conditions was discussed.
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© The Japanese Biochemical Society
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