Abstract
1. Spleen phosphodiesterase digestion of yeast RNA showed no critical change in its potency. The finding by Heppel that the active structure in pancreatic RNase-predigested RNA is resistant to this enzyme was confirmed.
2. Considerable rise in hemolysin-forming potency, such as shown in alkali hydrolysis, was detected during the hydrolysis of yeast RNA by Habuvenom phosphodiesterase. Pancreatic RNase-digested RNA was scarcely affected by this enzyme digestion.
3. Potato phosphodiesterase digestion manifested the effect similar to that of spleen phosphodiesterase digestion on the RNase-predigested RNA. Hydrolysates were separated by means of paper chromatography with isopropanol-water-ammonia system and potency was found only as the starting position.
4. During digestion with RNase of Aspergillus oiyziae, unlike other phosphodiesterase, potency of the yeast RNA and pancreatic RNase-digested RNA diminished very rapidly.
5. Prostatic phosphomonoesterase digestion had no critical erect on the potency of RNA.
6. Some discussions were given as to the possible structure of the active substance.
The authors are indebted to Prof. F. Egami for the interest he has taken and the encouragement he has given during this work.
