1959 Volume 46 Issue 4 Pages 453-461
1. The action of various inhibitors on pyruvic oxidase system, cocar-boxylase, or thiamine was examined. The catalytic action of thiamine derivatives was also studied instead of thiamine in the reaction of acetoin formation.
2. Thiamine combined with PCMB was detected and analyzed. It could be concluded that PCMB combines with the thiol group of thiamine.
3. From the experimental results the active center of thiamine or TDP was discussed. It was concluded that -SH, -NH2, and thiazole-N were possibly responsible for their catalytic action.
The author wishes to express his gratitude to Prof. N. Shimazono and Dr. N. Hosoya for their encouragement and helpful suggestions.