THE STRUCTURE OF SOME PEPTIDES IN TAKA-AMYLASE A

Abstract

1. The partial hydrolysates of DNP-Taka-amylase A were investigated.
2. Two fractions were obtained by using the adsorption chromatography with talk-celite column and with activated carbon.
Each fraction was further fractionated by countercurrent distribution method combined with celite or silica gel chromatography.
3. The structure of three peptides were elucidated to be valyl-(lysine, glutamic acid)-aspartic acid, phenylalanyl-aspartic acid and leucyl-aspartyl-tyrosine.
The authors are grateful to Sankyo Co., Ltd. for supplying them ‘Takadiastase Sankyo’.