1959 Volume 46 Issue 6 Pages 695-704
1. It was confirmed that the carbohydrates found in Taka-amylase A constitute an integral part of the enzyme molecule.
2. All the carbohydrate components except for the hexosamines seem to be linked to the enzyme proteins through the hydroxyl group of a particular serine residue.
3. In the glycopeptides, isolated from the partial hydrolysates of Taka-amylase A, one mole of xylose and 7-8 moles of mannose are present per one amino terminal residue.
4. The amino acid sequence of the glycopeptide was suggested to be seryl-glutamyl-aspartyl-glycyl-(alanine, threonine).
The authors wish to express their gratitudes to Sankyo Co., Ltd. for their kind supply of ‘Takadiastase Sankyo’ and to Dr. T. Ikenaka for his valuable advice during the course of this research